Defining the anti-Shine-Dalgarno sequence interaction and quantifying its functional role in regulating translation efficiency
نویسندگان
چکیده
Interdepartmental Program in Biological Sciences, Northwestern University, Evanston, IL, 60208, USA Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208, USA Northwestern Institute on Complex Systems, Northwestern University, Evanston, IL, 60208, USA Kellogg School of Management, Northwestern University, Evanston, IL, 60208, USA Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, 60208, USA Department of Physics and Astronomy, Northwestern University, Evanston, IL, 60208, USA Howard Hughes Medical Institute, Northwestern University, Evanston, IL, 60208, USA
منابع مشابه
Listeria Monocytogenes La111 and Klebsiella Pneumoniae KCTC 2242: Shine-Dalgarno Sequences
Listeria monocytogenes can cause serious infection and recently, relapse of listeriosis has been reported in leukemia and colorectal cancer, and the patients with Klebsiella pneumoniae are at increased risk of colorectal cancer. Translation initiation codon recognition is basically mediated by Shine-Dalgarno (SD) and the anti-SD sequences at the small ribosomal RNA (ssu rRNA). In this research,...
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Studies dating back to the 1970s established that sequence complementarity between the anti-Shine-Dalgarno (aSD) sequence on prokaryotic ribosomes and the 5' untranslated region of mRNAs helps to facilitate translation initiation. The optimal location of aSD sequence binding relative to the start codon, the full extents of the aSD sequence and the functional form of the relationship between aSD...
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Replication of the IncB miniplasmid pMU720 requires synthesis of the replication initiator protein, RepA, whose translation is coupled to that of a leader peptide, RepB. The unusual feature of this system is that translational coupling in repBA has to be activated by the formation of a pseudoknot immediately upstream of the repA Shine-Dalgarno sequence. A small antisense RNA, RNAI, controls rep...
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Chloroplast mRNAs in Euglena gracilis fall into two classes. One class has a Shine-Dalgarno sequence 5' to the AUG start codon while the other group of mRNAs does not have any conserved sequence elements near the start codon. The chloroplast mRNA encoding the atpH gene has been selected as an example of a message which has a Shine-Dalgarno sequence (GGAGUU) located in the initiation region. Mut...
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